12172-1-AP

antibody from Invitrogen Antibodies

Targeting: FKBP10 FKBP6, FKBP65, FLJ20683, FLJ22041, FLJ23833, hFKBP65

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12172-1-AP

Provider

Invitrogen Antibodies

Product name

FKBP65 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: PFLAYGEKG YGTVIPPQAS LVFHVLLIDV HNPKDAVQLE TLELPPGCVR RAGAGDFMRY HYNGSLMDGT LFDSSYSRNH TYNTYIGQGY IIPGMDQGLQ GACMGERRRI TIPPHLAYGE NGTGDKIPGS AVLIFNVHVI DFHNPADVVE IRTLSRPSET CNETTKLGDF VRYHYNCSLL DGTQLFTSHD YGAPQEATLG ANKVIEGLDT GLQGMCVGER RQLIVPPHLA HGESGARGVP GSAVLLFEVE LVSREDGLPT GYLFVWHKDP PANLFEDMDL NKDGEVPPEE FSTFIKAQVS EGKGRLMPGQ DPEKTIGDMF QNQDRNQDGK ITVDELKLKS DEDEERVHEE L (233-582 aa encoded by BC016467)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 12172-1-AP (FKBP65 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12172-1-AP (FKBP65 antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-FKBP65 (IP:12172-1-AP, 4ug; Detection:12172-1-AP 1:600) with HEK-293 cells lysate 2800ug.