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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-62469 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RHPN2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DHQEKCLSQL YDHMPEGLTP LATLKNDQQR RQLGKSHLRR AMAHHEESVR EASLCKKLRS IEVLQKVLCA AQERSRLTYA QHQEEDDLLN L
- Concentration
- 0.1 mg/mL
Submitted references Overexpression of rhophilin 2 promotes pancreatic ductal adenocarcinoma.
Overexpression of Rhophilin Rho GTPase-binding protein 2 promotes hepatocellular carcinoma.
Function of miR-200a in proliferation and apoptosis of non-small cell lung cancer cells.
Bo W, Feng X, Tang X
Oncology letters 2021 Jan;21(1):76
Oncology letters 2021 Jan;21(1):76
Overexpression of Rhophilin Rho GTPase-binding protein 2 promotes hepatocellular carcinoma.
Yuan B, Bo W, Feng X, Hu Y, Zeng J
Oncology letters 2020 Dec;20(6):382
Oncology letters 2020 Dec;20(6):382
Function of miR-200a in proliferation and apoptosis of non-small cell lung cancer cells.
Huang Y, Bao T, Li Z, Ji G, Zhang L
Oncology letters 2020 Aug;20(2):1256-1262
Oncology letters 2020 Aug;20(2):1256-1262
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RHPN2 in human cell line A549 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a RHPN2 Polyclonal Antibody (Product # PA5-62469).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RHPN2 in human appendix using a RHPN2 Polyclonal Antibody (Product # PA5-62469) shows cytoplasmic and nuclear positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Hepatocellular carcinoma tissues display higher levels of RHPN2 mRNA and protein. (A) Representative images of hepatocellular carcinoma and adjacent normal tissues stained for RHPN2. (B) RHPN2 protein levels in 31 hepatocellular carcinoma samples and matched normal adjacent tissues were quantified. The RHPN2 protein levels in tumor and normal tissues were compared using paired two-tailed Student's t-tests. (C) Mean values of RHPN2 protein levels in hepatocellular carcinoma samples. (D) RHPN2 mRNA levels in hepatocellular carcinoma samples were analyzed using reverse transcription-quantitative PCR. The RHPN2 mRNAs levels in tumor and normal tissues were compared using paired two-tailed Student's t-tests. (E) Mean values of RHPN2 mRNA expression in 31 hepatocellular carcinoma tissues and their matched adjacent normal tissues. (F) Patient survival according to RHPN2 expression was analyzed by using log-rank test. *P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. PDAC tissues have higher levels of RHPN2 mRNA and protein levels compared with normal tissues. (A) Representative immunohistochemistry images of normal and tumor samples from patients with PDAC stained with RHPN2 (magnification, x200). RHPN2 expression in the cytoplasm was denoted by the blue arrow. (B) Quantification of RHPN2 mRNA levels in the 61 tumor and paired normal adjacent tissues from PDAC patients. (C) Mean values of RHPN2 mRNA expression in the 61 tumor and paired normal adjacent tissues from PDAC patients were calculated. Data are presented as mean +- SD. (D) Survival curves of RHPN2-high vs. RHPN2-low patients with PDAC across 50 months, with analysis performed by the log-rank test. Each experiment was repeated at least three times. *P
