- PA5-25538 - Invitrogen Antibodies CPEB4 antibody

Xie H, Wang J, Wang B
Cancer management and research 2021;13:5337-5350

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis in A375 cell lysates (15 µg per lane) using a CPEB4 polyclonal antibody (Product # PA5-25538).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis in mouse Neuro-2a cell lysates (15 µg per lane) using a CPEB4 polyclonal antibody (Product # PA5-25538).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human lung carcinoma using a CPEB4 polyclonal antibody (Product # PA5-25538), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 CPEB4 was a downstream target of miR-758-3p. ( A ) Bioinformatics databases were used to predict the potential target genes of miR-758-3p. ( B ) The expression of potential target genes was detected by qRT-PCR in normal tissues and cervical cancer tissues. ( C ) The complementary binding sites of miR-758-3p and CPEB4 3'UTR were shown. ( D - G ) Dual-luciferase reporter assay and RIP assay were performed to verify the interaction between miR-758-3p and CPEB. ( H and G ) The mRNA and protein expression of CPEB4 were respectively determined by qRT-PCR and Western blot in in SiHa and CaSki cells transfected with anti-miR-NC, anti-miR-758-3p, miR-NC, or miR-758-3p. ( J and K ) CPEB4 mRNA and protein expression in normal tissues and cervical cancer tissues were detected by qRT-PCR and Western blot, respectively. ( L ) The correlation between CPEB4 mRNA expression and miR-758-3p expression was analyzed in cervical cancer tissues. ( M and N ) The mRNA and protein levels of CPEB4 in ECT1/E6E7, SiHa and CaSki cells were measured by qRT-PCR and Western blot, respectively. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Overexpression of miR-758-3p repressed cervical cancer cell progression by downregulating CPEB4. ( A and B ) The mRNA and protein levels of CPEB4 in SiHa and CaSki cells transfected with pcDNA or pcDNA-CPEB4 were measured by qRT-PCR and Western blot, respectively. ( C - N ) SiHa and CaSki cells were transfected with miR-NC, miR-758-3p, miR-758-3p + pcDNA, miR-758-3p + pcDNA-CPEB4. ( C and D ) Cell viability was measured using CCK-8 assay. ( E and F ) Cell cycle distribution was measured using flow cytometry analysis. ( G ) Colony formation assay was performed to measure colony formation ability. ( H ) Edu assay was used to assess cell proliferation ability (x200). ( I and J ) Western blot assay was conducted to examine the protein levels of PCNA and Cyclin D1. ( K and L ) Cell migration and invasion were tested using transwell assay (x100). ( M and N ) The protein levels of E-cadherin, Snail and Vimentin were determined by Western blot assay. * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 Circ_0003221 regulated CPEB4 expression by sponging miR-758-3p. ( A ) The correlation between CPEB4 mRNA expression and circ_0003221 expression was analyzed in cervical cancer tissues. ( B and C ) SiHa and CaSki cells were transfected with si-NC, si-circ_0003221, si-circ_0003221 + anti-miR-NC, or si-circ_0003221 + anti-miR-758-3p. Then qRT-PCR and Western blot were used to measure the mRNA and protein expression of CPEB4. * P

PA5-25538