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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 16921-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLSPQRVAA AASRGADDAM ESSKPGPVQV VLVQKDQHSF ELDEKALASI LLQDHIRDLD VVVVSVAGAF RKGKSFILDF MLRYLYSQKE SGHSNWLGDP EEPLTGFSWR GGSDPETTGI QIWSEVFTVE KPGGKKVAVV LMDTQGAFDS QSTVKDCATI FALSTMTSSV QIYNLSQNIQ EDDLQQLQLF TEYGRLAMDE IFQKPFQTLM FLVRDWSFPY EYSYGLQGGM AFLDKRLQVK EHQHEEIQNV RNHIHSCFSD VTCFLLPHPG LQVATSPDFD GKLKDIAGEF KEQLQALIPY VLNPSKLMEK EINGSKVTCR GLLEYFKAYI KIYQGEDLPH PKSMLQATAE AN (1-351 aa encoded by BC077727)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16921-1-AP ( ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:1500) with HeLa cells lysate 3200ug.
