PA5-28486

antibody from Invitrogen Antibodies

Targeting: NODAL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28486

Provider

Invitrogen Antibodies

Product name

Nodal Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Human ESC.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nodal in Mouse tissue extract (50 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with Nodal Polyclonal antibody (Product # PA5-28486) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Nodal Polyclonal Antibody (Product # PA5-28486). Sample (20 µg of whole cell lysate). Lane A: Human ESC. 10% SDS PAGE. Nodal Polyclonal Antibody (Product # PA5-28486) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Nodal Polyclonal Antibody (Product # PA5-28486). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Nodal Polyclonal Antibody (Product # PA5-28486) diluted at 1:500.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA detection of NODAL using Nodal Polyclonal Antibody (Product # PA5-28486) for capture at a concentration of 5 µg/mL and a NODAL Polyclonal Antibody for detection at a concentration of 1.5 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Nodal was performed in paraffin-embedded rat brain tissue using Nodal Polyclonal Antibody (Product # PA5-28486) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Nodal (Product # PA5-28486) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.