Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF3025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TGF-beta RI/ALK-5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TGF-beta RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse TGF-beta RI is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P36897
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references POH1 contributes to hyperactivation of TGF-β signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-β receptors and caveolin-1.
MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells.
Key role of the endothelial TGF-β/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.
MiR-142-3p represses TGF-β-induced growth inhibition through repression of TGFβR1 in non-small cell lung cancer.
Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta.
Wang B, Xu X, Yang Z, Zhang L, Liu Y, Ma A, Xu G, Tang M, Jing T, Wu L, Liu Y
EBioMedicine 2019 Mar;41:320-332
EBioMedicine 2019 Mar;41:320-332
MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells.
Chen Y, Wang DD, Wu YP, Su D, Zhou TY, Gai RH, Fu YY, Zheng L, He QJ, Zhu H, Yang B
British journal of cancer 2017 Oct 10;117(8):1192-1201
British journal of cancer 2017 Oct 10;117(8):1192-1201
Key role of the endothelial TGF-β/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.
Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S
PloS one 2014;9(6):e100310
PloS one 2014;9(6):e100310
MiR-142-3p represses TGF-β-induced growth inhibition through repression of TGFβR1 in non-small cell lung cancer.
Lei Z, Xu G, Wang L, Yang H, Liu X, Zhao J, Zhang HT
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jun;28(6):2696-704
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jun;28(6):2696-704
Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
Yao Y, Zebboudj AF, Torres A, Shao E, Boström K
Cardiovascular research 2007 May 1;74(2):279-89
Cardiovascular research 2007 May 1;74(2):279-89
Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta.
Haynes LP, Fitzgerald DJ, Wareing B, O'Callaghan DW, Morgan A, Burgoyne RD
Proteomics 2006 Mar;6(6):1822-32
Proteomics 2006 Mar;6(6):1822-32
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TGF-beta RI/ALK-5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TGF-beta RI/ALK-5 in Human PBMCs. TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.