AMAb90690

antibody from Atlas Antibodies

Targeting: PBRM1 BAF180, PB1

Western blot (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb90690

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90690, RRID:AB_2665633

Product name

Anti-PBRM1

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

KMEEYDDVNLLTADFQLLFNNAKSYYKPDSPEYKA
ACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQG
TVTEGSSPAYLKEILEQLLEAIVVATNPSGRLISE
LFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGS
YKSIHA


Isotype

IgG

Antibody clone number

CL0331

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PBRM1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa]Lane 2: Human cell line RT-4

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human endometrium and skeletal muscle tissues using AMAb90690 antibody. Corresponding PBRM1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human renal cancer shows nuclear immunoreactivity in a subset of cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast cancer shows nuclear positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach cancer shows nuclear immunoreactivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows nuclear positivity in both tubuli and glomeruli.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows absence of nuclear staining in hepatocytes (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney cancer shows moderate to strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.