H00056937-M01

antibody from Novus Biologicals

Targeting: PMEPA1 STAG1, TMEPAI

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

H00056937-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00056937-M01, RRID:AB_2166714

Product name

Mouse Monoclonal TMEPAI Antibody

Antibody type

Monoclonal

Description

IgG purified. TMEPAI - transmembrane, prostate androgen induced RNA

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Loss of miR-449a in ERG-associated prostate cancer promotes the invasive phenotype by inducing SIRT1.

Kumar P, Sharad S, Petrovics G, Mohamed A, Dobi A, Sreenath TL, Srivastava S, Biswas R
Oncotarget 2016 Apr 19;7(16):22791-806

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Silencing of PMEPA1 accelerates the growth of prostate cancer cells through AR, NEDD4 and PTEN.

Li H, Mohamed AA, Sharad S, Umeda E, Song Y, Young D, Petrovics G, McLeod DG, Sesterhenn IA, Sreenath T, Dobi A, Srivastava S
Oncotarget 2015 Jun 20;6(17):15137-49

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Mutation or loss of p53 differentially modifies TGFβ action in ovarian cancer.

Ó hAinmhire E, Quartuccio SM, Cheng W, Ahmed RA, King SM, Burdette JE
PloS one 2014;9(2):e89553

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TGF-β induced TMEPAI/PMEPA1 inhibits canonical Smad signaling through R-Smad sequestration and promotes non-canonical PI3K/Akt signaling by reducing PTEN in triple negative breast cancer.

Singha PK, Pandeswara S, Geng H, Lan R, Venkatachalam MA, Saikumar P
Genes & cancer 2014 Sep;5(9-10):320-36

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Identification of two distinct carcinoma-associated fibroblast subtypes with differential tumor-promoting abilities in oral squamous cell carcinoma.

Costea DE, Hills A, Osman AH, Thurlow J, Kalna G, Huang X, Pena Murillo C, Parajuli H, Suliman S, Kulasekara KK, Johannessen AC, Partridge M
Cancer research 2013 Jul 1;73(13):3888-901

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TMEPAI regulates EMT in lung cancer cells by modulating the ROS and IRS-1 signaling pathways.

Hu Y, He K, Wang D, Yuan X, Liu Y, Ji H, Song J
Carcinogenesis 2013 Aug;34(8):1764-72

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TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling.

Watanabe Y, Itoh S, Goto T, Ohnishi E, Inamitsu M, Itoh F, Satoh K, Wiercinska E, Yang W, Shi L, Tanaka A, Nakano N, Mommaas AM, Shibuya H, Ten Dijke P, Kato M
Molecular cell 2010 Jan 15;37(1):123-34

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Transforming growth factor-beta (TGF-beta)-inducible gene TMEPAI converts TGF-beta from a tumor suppressor to a tumor promoter in breast cancer.

Singha PK, Yeh IT, Venkatachalam MA, Saikumar P
Cancer research 2010 Aug 1;70(15):6377-83

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A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells.

Li H, Xu LL, Masuda K, Raymundo E, McLeod DG, Dobi A, Srivastava S
The Journal of biological chemistry 2008 Oct 24;283(43):28988-95

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High level expression of STAG1/PMEPA1 in an androgen-independent prostate cancer PC3 subclone.

Hirokawa YS, Takagi A, Uchida K, Kozuka Y, Yoneda M, Watanabe M, Shiraishi T
Cellular & molecular biology letters 2007 Sep;12(3):370-7

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: TMEPAI Antibody (2A12) [H00056937-M01] - Detection limit for recombinant GST tagged TMEPAI is approximately 1ng/ml as a capture antibody.