Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-89702 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89702, RRID:AB_11007565
- Product name
- Rabbit Polyclonal Frizzled-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Frizzled-6 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Functional dissection of the N-terminal extracellular domains of Frizzled 6 reveals their roles for receptor localization and Dishevelled recruitment.
Valnohova J, Kowalski-Jahn M, Sunahara RK, Schulte G
The Journal of biological chemistry 2018 Nov 16;293(46):17875-17887
The Journal of biological chemistry 2018 Nov 16;293(46):17875-17887
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Frizzled-6 Antibody [NBP1-89702] - Analysis in control (vector only transfected HEK293T lysate) and FZD6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Frizzled-6 Antibody [NBP1-89702] - Analysis in human cell line A-431 and human cell line CACO-2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human urinary bladder shows strong positivity in urothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human placenta shows moderate cytoplasmic and membranous positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NBP1-89702] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.