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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005863-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005863-M02, RRID:AB_2179360
- Product name
- Mouse Monoclonal RGL2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RGL2 - ral guanine nucleotide dissociation stimulator-like 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Localization of RalB signaling at endomembrane compartments and its modulation by autophagy.
M-Ras induces Ral and JNK activation to regulate MEK/ERK-independent gene expression in MCF-7 breast cancer cells.
Aberrant overexpression of the Rgl2 Ral small GTPase-specific guanine nucleotide exchange factor promotes pancreatic cancer growth through Ral-dependent and Ral-independent mechanisms.
Singh MK, Martin APJ, Joffre C, Zago G, Camonis J, Coppey M, Parrini MC
Scientific reports 2019 Jun 20;9(1):8910
Scientific reports 2019 Jun 20;9(1):8910
M-Ras induces Ral and JNK activation to regulate MEK/ERK-independent gene expression in MCF-7 breast cancer cells.
Castro AF, Campos T, Babcock JT, Armijo ME, MartÃnez-Conde A, Pincheira R, Quilliam LA
Journal of cellular biochemistry 2012 Apr;113(4):1253-64
Journal of cellular biochemistry 2012 Apr;113(4):1253-64
Aberrant overexpression of the Rgl2 Ral small GTPase-specific guanine nucleotide exchange factor promotes pancreatic cancer growth through Ral-dependent and Ral-independent mechanisms.
Vigil D, Martin TD, Williams F, Yeh JJ, Campbell SL, Der CJ
The Journal of biological chemistry 2010 Nov 5;285(45):34729-40
The Journal of biological chemistry 2010 Nov 5;285(45):34729-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RGL2 Antibody (4D10) [H00005863-M02] - RGL2 monoclonal antibody (M02), clone 4D10 Analysis of RGL2 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RGL2 Antibody (4D10) [H00005863-M02] - Detection limit for recombinant GST tagged RGL2 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RGL2 Antibody (4D10) [H00005863-M02] - Analysis of monoclonal antibody to RGL2 on HeLa cell. Antibody concentration 10 ug/ml.
