Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP2-31896 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal EHD3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human EHD3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references In Vivo Developmental Trajectories of Human Podocyte Inform In Vitro Differentiation of Pluripotent Stem Cell-Derived Podocytes.
Tran T, Lindström NO, Ransick A, De Sena Brandine G, Guo Q, Kim AD, Der B, Peti-Peterdi J, Smith AD, Thornton M, Grubbs B, McMahon JA, McMahon AP
Developmental cell 2019 Jul 1;50(1):102-116.e6
Developmental cell 2019 Jul 1;50(1):102-116.e6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EHD3 Antibody [NBP2-31896] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EHD3 Antibody [NBP2-31896] - Analysis in human cell line NTERA-2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31896] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31896] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31896] - Staining of human liver shows moderate membranous positivity in hepatic sinusoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31896] - Staining of human lymphoid tissues shows moderate positivity in reticular fibers in germinal center cells.