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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00001212-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001212-M01, RRID:AB_537809
- Product name
- Mouse Monoclonal CLTB Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CLTB - clathrin, light polypeptide (Lcb)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Crosstalk between CLCb/Dyn1-Mediated Adaptive Clathrin-Mediated Endocytosis and Epidermal Growth Factor Receptor Signaling Increases Metastasis.
Chen PH, Bendris N, Hsiao YJ, Reis CR, Mettlen M, Chen HY, Yu SL, Schmid SL
Developmental cell 2017 Feb 6;40(3):278-288.e5
Developmental cell 2017 Feb 6;40(3):278-288.e5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLTB Antibody (4B12-1E3) [H00001212-M01] - Analysis of CLTB expression in transfected 293T cell line by CLTB monoclonal antibody (M01), clone 4B12-1E3.Lane 1: CLTB transfected lysate(23.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLTB Antibody (4B12-1E3) [H00001212-M01] - CLTB monoclonal antibody (M01), clone 4B12-1E3 Analysis of CLTB expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CLTB Antibody (4B12-1E3) [H00001212-M01] - Detection limit for recombinant GST tagged CLTB is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CLTB Antibody (4B12-1E3) [H00001212-M01] - Analysis of monoclonal antibody to CLTB on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLTB Antibody (4B12-1E3) [H00001212-M01] - Analysis of monoclonal antibody to CLTB on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 2 ug/ml.
