10455-1-AP

antibody from Invitrogen Antibodies

Targeting: CLTB Lcb

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10455-1-AP

Provider

Invitrogen Antibodies

Product name

CLTB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MADDFGFFS SSESGAPEAA EEDPAAAFLA QQESEIAGIE NDEGFGAPAG SHAAPAQPGP TSGAGSEDMG TTVNGDVFQE ANGPADGYAA IAQADRLTQE PESIRKWREE QRKRLQELDA ASKVTEQEWR EKAKKDLEEW NQRQSEQVEK NKINNRASEE AFVKESKEET PGTEWEKVAQ LCDFNPKSSK QCKDVSRLRS VLMSLKQTPL SR (1-211 aa encoded by BC006457)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10455-1-AP (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 10455-1-AP (CLTB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10455-1-AP (CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 10455-1-AP (CLTB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HEK-293 cells using 10455-1-AP (CLTB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CLTB (IP:10455-1-AP, 4ug; Detection:10455-1-AP 1:500) with mouse brain tissue lysate 4000ug.