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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21887 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETEA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299, HeLa. Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.57 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ETEA Polyclonal Antibody (Product # PA5-21887). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Hela. 10% SDS PAGE. ETEA Polyclonal Antibody (Product # PA5-21887) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ETEA showing staining in the endoplasmic reticulum of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an ETEA polyclonal antibody (Product # PA5-21887) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETEA Polyclonal Antibody detects FAF2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAF2 protein stained by ETEA Polyclonal Antibody (Product # PA5-21887) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETEA Polyclonal Antibody detects ETEA protein at cytoplasm on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ETEA Polyclonal Antibody (Product # PA5-21887) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
