PAB28636

antibody from Abnova Corporation

Targeting: TOMM22 TOM22

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28636

Provider

Abnova Corporation

Product name

TOMM22 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TOMM22.

Antigen sequence

MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEED
DDEELDETLSERLWGLTEMFPERVRSAAGATFDLS
LFVAQK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp with TOMM22 polyclonal antibody (Cat # PAB28636) at 1:100-1:250 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with TOMM22 polyclonal antibody (Cat # PAB28636) at 1:100-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with TOMM22 polyclonal antibody (Cat # PAB28636) at 1-4 ug/ml shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with TOMM22 polyclonal antibody (Cat # PAB28636) shows strong cytoplasmic positivity in renal tubules at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)