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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- GTX10436 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX10436, RRID:AB_381042
- Product name
- TOMM22 antibody [1C9-2]
- Antibody type
- Monoclonal
- Reactivity
- Human, Simian
- Host
- Mouse
Submitted references Bacterial porin disrupts mitochondrial membrane potential and sensitizes host cells to apoptosis.
Kozjak-Pavlovic V, Dian-Lothrop EA, Meinecke M, Kepp O, Ross K, Rajalingam K, Harsman A, Hauf E, Brinkmann V, Günther D, Herrmann I, Hurwitz R, Rassow J, Wagner R, Rudel T
PLoS pathogens 2009 Oct;5(10):e1000629
PLoS pathogens 2009 Oct;5(10):e1000629
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Co-staining of mitochondria and ER-Golgi intermediate compartment in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 2 μg/mL Mouse Anti-TOM22, Atto 488 Conjμgate (green) and 1 μg/mL Rabbit Anti-ERGIC-53/p58, Cy3 Conjμgate (ER-Golgi intermediate compartment stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.
