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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 720364 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AVPR1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Correlation between miRNA target site polymorphisms in the 3' UTR of AVPR1A and the risk of hypertension in the Chinese Han population.
Zhang L, Liu J, Cheng P, Lv F
Bioscience reports 2019 May 31;39(5)
Bioscience reports 2019 May 31;39(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK 293 cells were transiently transfected with vasopressin receptor 1A/AVPR1A or Bradykinin receptor B2/BDKRB2. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-vasopressin receptor 1A/AVPR1A Rabbit Polyclonal Antibody (Product # 720364 at a concentration of 2 µg/mL). Bands from 65-80 kDa corresponding to vasopressin receptor 1A/AVPR1A was observed.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK 293 cells were transiently transfected with vasopressin receptor 1A/AVPR1A or Bradykinin receptor B2/BDKRB2. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-vasopressin receptor 1A/AVPR1A Rabbit Polyclonal Antibody (Product # 720364 at a concentration of 2 µg/mL). Bands from 65-80 kDa corresponding to vasopressin receptor 1A/AVPR1A was observed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sections of human pancreas were dewaxed, microwaved in citric acid and incubated with Anti-vasopressin receptor 1A/AVPR1A Rabbit Polyclonal Antibody (Product # 720364 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of pancreatic islets is completely neutralized by preincubation of the antibody with its immunizing peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sections of human kidney were dewaxed, microwaved in citric acid and incubated with Anti-vasopressin receptor 1A/AVPR1A Rabbit Polyclonal Antibody (Product # 720364 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of kidney tubules is completely neutralized by preincubation of the antibody with its immunizing peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 AVPR1A protein expression by western blotting The loading quantity of protein sample was 30 ug. Blank indicates that the cells were transfected with 100 ng ssEGFP miRNA. Lane 1: AVPR1A rs11174811 CCVE type HUVEC line, lane 2: AVPR1A rs11174811 CA/AA genotype HUVEC line, lane 4 and 6: AVPR1A rs3803107 GG genotype HUVECs. Lane 5 and 7: AVPR1A rs3803107 GA/AA genotype of HUVEC line.
