Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-14884 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCN5 Monoclonal Antibody (S.775.4)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Antibody clone number
- S.775.4
- Concentration
- 134 µg/mL
Submitted references LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling.
Huang X, Pan L, Zuo Z, Li M, Zeng L, Li R, Ye Y, Zhang J, Wu G, Bai R, Zhuang L, Wei L, Zheng Y, Su J, Deng J, Deng S, Zhang S, Zhu S, Che X, Wang C, Wu C, Chen R, Lin D, Zheng J
Nature communications 2021 Jun 22;12(1):3830
Nature communications 2021 Jun 22;12(1):3830
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of GCN5 was achieved by transfecting HeLa cells with GCN5 specific siRNAs (Silencer® select Product # s5657). Western blot analysis (Fig. a) was performed using whole cell extracts from the GCN5 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GCN5 Monoclonal Antibody (Product # MA5-14884, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GCN5.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of Hep G2 (Lane 1), Raji (Lane 2), Ramos (Lane 3), U-2OS (Lane 4), A549 (Lane 5), SH-SY5Y (Lane 6), HeLa (Lane 7) and HCT 116 (Lane 8). The blot was probed with Anti-GCN5 Monoclonal Antibody (S.775.4) (Product # MA5-14884, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 94 kDa band corresponding to GCN5 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GCN5L2 in HeLa cells using a GCN5L2 monoclonal antibody (Product # MA5-14884) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
