Antibody data
- Antibody Data
- Antigen structure
- References [47]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 14276-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14276-1-AP, RRID:AB_10888627
- Product name
- CNOT1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CNOT1 antibody (Cat. #14276-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain.
Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex.
YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.
Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.
RNF219 interacts with CCR4-NOT in regulating stem cell differentiation.
Context-dependent functional compensation between Ythdf m6A reader proteins.
Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis.
Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.
Regulation of Fetal Genes by Transitions among RNA-Binding Proteins during Liver Development.
CSDE1 controls gene expression through the miRNA-mediated decay machinery.
UPF1/SMG7-dependent microRNA-mediated gene regulation.
A human immune dysregulation syndrome characterized by severe hyperinflammation with a homozygous nonsense Roquin-1 mutation.
Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.
Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.
Degradation of a Novel DNA Damage Response Protein, Tankyrase 1 Binding Protein 1, following Adenovirus Infection.
Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA.
Translational control of ERK signaling through miRNA/4EHP-directed silencing.
IMP2 and IMP3 cooperate to promote the metastasis of triple-negative breast cancer through destabilization of progesterone receptor.
Human MARF1 is an endoribonuclease that interacts with the DCP1:2 decapping complex and degrades target mRNAs.
CNOT1 is involved in TTP‑mediated ICAM‑1 and IL‑8 mRNA decay.
Comprehensive Identification of Nuclear and Cytoplasmic TNRC6A-Associating Proteins.
Cap-binding protein 4EHP effects translation silencing by microRNAs.
DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.
Transcriptional Regulator CNOT3 Defines an Aggressive Colorectal Cancer Subtype.
Alternative splicing of CNOT7 diversifies CCR4-NOT functions.
Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
CNOT1 cooperates with LMNA to aggravate osteosarcoma tumorigenesis through the Hedgehog signaling pathway.
CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.
A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1.
Recruitment of the 4EHP-GYF2 cap-binding complex to tetraproline motifs of tristetraprolin promotes repression and degradation of mRNAs with AU-rich elements.
Codon Usage and 3' UTR Length Determine Maternal mRNA Stability in Zebrafish.
Post-transcriptional Control of Tumor Cell Autonomous Metastatic Potential by CCR4-NOT Deadenylase CNOT7.
The cAMP pathway regulates mRNA decay through phosphorylation of the RNA-binding protein TIS11b/BRF1.
YTHDF2 destabilizes m(6)A-containing RNA through direct recruitment of the CCR4-NOT deadenylase complex.
CNOT3 suppression promotes necroptosis by stabilizing mRNAs for cell death-inducing proteins.
A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C.
The eIF4E-Binding Protein 4E-T Is a Component of the mRNA Decay Machinery that Bridges the 5' and 3' Termini of Target mRNAs.
ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway.
Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
CNOT7/hCAF1 is involved in ICAM-1 and IL-8 regulation by tristetraprolin.
Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis.
Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.
Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.
Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs.
The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex.
Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.
Hsieh HH, Chen YA, Chang YJ, Wang HH, Yu YH, Lin SW, Huang YJ, Lin S, Chang CJ
Journal of inflammation (London, England) 2021 Jun 5;18(1):22
Journal of inflammation (London, England) 2021 Jun 5;18(1):22
Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex.
Munakata F, Suzawa M, Ui-Tei K
Genes 2021 Feb 13;12(2)
Genes 2021 Feb 13;12(2)
YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.
Liu J, Gao M, Xu S, Chen Y, Wu K, Liu H, Wang J, Yang X, Wang J, Liu W, Bao X, Chen J
Cell reports 2020 Sep 8;32(10):108120
Cell reports 2020 Sep 8;32(10):108120
Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.
Jalihal AP, Pitchiaya S, Xiao L, Bawa P, Jiang X, Bedi K, Parolia A, Cieslik M, Ljungman M, Chinnaiyan AM, Walter NG
Molecular cell 2020 Sep 17;79(6):978-990.e5
Molecular cell 2020 Sep 17;79(6):978-990.e5
RNF219 interacts with CCR4-NOT in regulating stem cell differentiation.
Du H, Chen C, Wang Y, Yang Y, Che Z, Liu X, Meng S, Guo C, Xu M, Fang H, Wang F, Lin C, Luo Z
Journal of molecular cell biology 2020 Oct 26;12(11):894-905
Journal of molecular cell biology 2020 Oct 26;12(11):894-905
Context-dependent functional compensation between Ythdf m6A reader proteins.
Lasman L, Krupalnik V, Viukov S, Mor N, Aguilera-Castrejon A, Schneir D, Bayerl J, Mizrahi O, Peles S, Tawil S, Sathe S, Nachshon A, Shani T, Zerbib M, Kilimnik I, Aigner S, Shankar A, Mueller JR, Schwartz S, Stern-Ginossar N, Yeo GW, Geula S, Novershtern N, Hanna JH
Genes & development 2020 Oct 1;34(19-20):1373-1391
Genes & development 2020 Oct 1;34(19-20):1373-1391
Systematic Analysis of Targets of Pumilio-Mediated mRNA Decay Reveals that PUM1 Repression by DNA Damage Activates Translesion Synthesis.
Yamada T, Imamachi N, Imamura K, Taniue K, Kawamura T, Suzuki Y, Nagahama M, Akimitsu N
Cell reports 2020 May 5;31(5):107542
Cell reports 2020 May 5;31(5):107542
Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.
Mostafa D, Takahashi A, Yanagiya A, Yamaguchi T, Abe T, Kureha T, Kuba K, Kanegae Y, Furuta Y, Yamamoto T, Suzuki T
RNA biology 2020 Mar;17(3):403-416
RNA biology 2020 Mar;17(3):403-416
Regulation of Fetal Genes by Transitions among RNA-Binding Proteins during Liver Development.
Suzuki T, Adachi S, Kikuguchi C, Shibata S, Nishijima S, Kawamoto Y, Iizuka Y, Koseki H, Okano H, Natsume T, Yamamoto T
International journal of molecular sciences 2020 Dec 7;21(23)
International journal of molecular sciences 2020 Dec 7;21(23)
CSDE1 controls gene expression through the miRNA-mediated decay machinery.
Kakumani PK, Harvey LM, Houle F, Guitart T, Gebauer F, Simard MJ
Life science alliance 2020 Apr;3(4)
Life science alliance 2020 Apr;3(4)
UPF1/SMG7-dependent microRNA-mediated gene regulation.
Park J, Seo JW, Ahn N, Park S, Hwang J, Nam JW
Nature communications 2019 Sep 13;10(1):4181
Nature communications 2019 Sep 13;10(1):4181
A human immune dysregulation syndrome characterized by severe hyperinflammation with a homozygous nonsense Roquin-1 mutation.
Tavernier SJ, Athanasopoulos V, Verloo P, Behrens G, Staal J, Bogaert DJ, Naesens L, De Bruyne M, Van Gassen S, Parthoens E, Ellyard J, Cappello J, Morris LX, Van Gorp H, Van Isterdael G, Saeys Y, Lamkanfi M, Schelstraete P, Dehoorne J, Bordon V, Van Coster R, Lambrecht BN, Menten B, Beyaert R, Vinuesa CG, Heissmeyer V, Dullaers M, Haerynck F
Nature communications 2019 Oct 21;10(1):4779
Nature communications 2019 Oct 21;10(1):4779
Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.
Pitchiaya S, Mourao MDA, Jalihal AP, Xiao L, Jiang X, Chinnaiyan AM, Schnell S, Walter NG
Molecular cell 2019 May 2;74(3):521-533.e6
Molecular cell 2019 May 2;74(3):521-533.e6
Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.
Park OH, Ha H, Lee Y, Boo SH, Kwon DH, Song HK, Kim YK
Molecular cell 2019 May 2;74(3):494-507.e8
Molecular cell 2019 May 2;74(3):494-507.e8
Degradation of a Novel DNA Damage Response Protein, Tankyrase 1 Binding Protein 1, following Adenovirus Infection.
Chalabi Hagkarim N, Ryan EL, Byrd PJ, Hollingworth R, Shimwell NJ, Agathanggelou A, Vavasseur M, Kolbe V, Speiseder T, Dobner T, Stewart GS, Grand RJ
Journal of virology 2018 Jun 15;92(12)
Journal of virology 2018 Jun 15;92(12)
Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA.
Rehage N, Davydova E, Conrad C, Behrens G, Maiser A, Stehklein JE, Brenner S, Klein J, Jeridi A, Hoffmann A, Lee E, Dianzani U, Willemsen R, Feederle R, Reiche K, Hackermüller J, Leonhardt H, Sharma S, Niessing D, Heissmeyer V
Nature communications 2018 Jan 19;9(1):299
Nature communications 2018 Jan 19;9(1):299
Translational control of ERK signaling through miRNA/4EHP-directed silencing.
Jafarnejad SM, Chapat C, Matta-Camacho E, Gelbart IA, Hesketh GG, Arguello M, Garzia A, Kim SH, Attig J, Shapiro M, Morita M, Khoutorsky A, Alain T, Gkogkas CG, Stern-Ginossar N, Tuschl T, Gingras AC, Duchaine TF, Sonenberg N
eLife 2018 Feb 7;7
eLife 2018 Feb 7;7
IMP2 and IMP3 cooperate to promote the metastasis of triple-negative breast cancer through destabilization of progesterone receptor.
Kim HY, Ha Thi HT, Hong S
Cancer letters 2018 Feb 28;415:30-39
Cancer letters 2018 Feb 28;415:30-39
Human MARF1 is an endoribonuclease that interacts with the DCP1:2 decapping complex and degrades target mRNAs.
Nishimura T, Fakim H, Brandmann T, Youn JY, Gingras AC, Jinek M, Fabian MR
Nucleic acids research 2018 Dec 14;46(22):12008-12021
Nucleic acids research 2018 Dec 14;46(22):12008-12021
CNOT1 is involved in TTP‑mediated ICAM‑1 and IL‑8 mRNA decay.
Shi JX, Li JS, Hu R, Zhao XC, Liang CC, Li XM, Wang H, Shi Y, Su X
Molecular medicine reports 2018 Aug;18(2):2321-2327
Molecular medicine reports 2018 Aug;18(2):2321-2327
Comprehensive Identification of Nuclear and Cytoplasmic TNRC6A-Associating Proteins.
Suzawa M, Noguchi K, Nishi K, Kozuka-Hata H, Oyama M, Ui-Tei K
Journal of molecular biology 2017 Oct 27;429(21):3319-3333
Journal of molecular biology 2017 Oct 27;429(21):3319-3333
Cap-binding protein 4EHP effects translation silencing by microRNAs.
Chapat C, Jafarnejad SM, Matta-Camacho E, Hesketh GG, Gelbart IA, Attig J, Gkogkas CG, Alain T, Stern-Ginossar N, Fabian MR, Gingras AC, Duchaine TF, Sonenberg N
Proceedings of the National Academy of Sciences of the United States of America 2017 May 23;114(21):5425-5430
Proceedings of the National Academy of Sciences of the United States of America 2017 May 23;114(21):5425-5430
DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T
Nature 2017 Mar 23;543(7646):568-572
Nature 2017 Mar 23;543(7646):568-572
The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.
Simsek D, Tiu GC, Flynn RA, Byeon GW, Leppek K, Xu AF, Chang HY, Barna M
Cell 2017 Jun 1;169(6):1051-1065.e18
Cell 2017 Jun 1;169(6):1051-1065.e18
Transcriptional Regulator CNOT3 Defines an Aggressive Colorectal Cancer Subtype.
Cejas P, Cavazza A, Yandava CN, Moreno V, Horst D, Moreno-Rubio J, Burgos E, Mendiola M, Taing L, Goel A, Feliu J, Shivdasani RA
Cancer research 2017 Feb 1;77(3):766-779
Cancer research 2017 Feb 1;77(3):766-779
Alternative splicing of CNOT7 diversifies CCR4-NOT functions.
Chapat C, Chettab K, Simonet P, Wang P, De La Grange P, Le Romancer M, Corbo L
Nucleic acids research 2017 Aug 21;45(14):8508-8523
Nucleic acids research 2017 Aug 21;45(14):8508-8523
Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
Hicks JA, Li L, Matsui M, Chu Y, Volkov O, Johnson KC, Corey DR
Cell reports 2017 Aug 15;20(7):1543-1552
Cell reports 2017 Aug 15;20(7):1543-1552
CNOT1 cooperates with LMNA to aggravate osteosarcoma tumorigenesis through the Hedgehog signaling pathway.
Cheng DD, Li J, Li SJ, Yang QC, Fan CY
Molecular oncology 2017 Apr;11(4):388-404
Molecular oncology 2017 Apr;11(4):388-404
CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.
Zheng X, Yang P, Lackford B, Bennett BD, Wang L, Li H, Wang Y, Miao Y, Foley JF, Fargo DC, Jin Y, Williams CJ, Jothi R, Hu G
Stem cell reports 2016 Nov 8;7(5):897-910
Stem cell reports 2016 Nov 8;7(5):897-910
A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1.
Geissler R, Simkin A, Floss D, Patel R, Fogarty EA, Scheller J, Grimson A
Genes & development 2016 May 1;30(9):1070-85
Genes & development 2016 May 1;30(9):1070-85
Recruitment of the 4EHP-GYF2 cap-binding complex to tetraproline motifs of tristetraprolin promotes repression and degradation of mRNAs with AU-rich elements.
Fu R, Olsen MT, Webb K, Bennett EJ, Lykke-Andersen J
RNA (New York, N.Y.) 2016 Mar;22(3):373-82
RNA (New York, N.Y.) 2016 Mar;22(3):373-82
Codon Usage and 3' UTR Length Determine Maternal mRNA Stability in Zebrafish.
Mishima Y, Tomari Y
Molecular cell 2016 Mar 17;61(6):874-85
Molecular cell 2016 Mar 17;61(6):874-85
Post-transcriptional Control of Tumor Cell Autonomous Metastatic Potential by CCR4-NOT Deadenylase CNOT7.
Faraji F, Hu Y, Yang HH, Lee MP, Winkler GS, Hafner M, Hunter KW
PLoS genetics 2016 Jan;12(1):e1005820
PLoS genetics 2016 Jan;12(1):e1005820
The cAMP pathway regulates mRNA decay through phosphorylation of the RNA-binding protein TIS11b/BRF1.
Rataj F, Planel S, Desroches-Castan A, Le Douce J, Lamribet K, Denis J, Feige JJ, Cherradi N
Molecular biology of the cell 2016 Dec 1;27(24):3841-3854
Molecular biology of the cell 2016 Dec 1;27(24):3841-3854
YTHDF2 destabilizes m(6)A-containing RNA through direct recruitment of the CCR4-NOT deadenylase complex.
Du H, Zhao Y, He J, Zhang Y, Xi H, Liu M, Ma J, Wu L
Nature communications 2016 Aug 25;7:12626
Nature communications 2016 Aug 25;7:12626
CNOT3 suppression promotes necroptosis by stabilizing mRNAs for cell death-inducing proteins.
Suzuki T, Kikuguchi C, Sharma S, Sasaki T, Tokumasu M, Adachi S, Natsume T, Kanegae Y, Yamamoto T
Scientific reports 2015 Oct 6;5:14779
Scientific reports 2015 Oct 6;5:14779
A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C.
Cano F, Rapiteanu R, Sebastiaan Winkler G, Lehner PJ
Nature communications 2015 Oct 16;6:8670
Nature communications 2015 Oct 16;6:8670
The eIF4E-Binding Protein 4E-T Is a Component of the mRNA Decay Machinery that Bridges the 5' and 3' Termini of Target mRNAs.
Nishimura T, Padamsi Z, Fakim H, Milette S, Dunham WH, Gingras AC, Fabian MR
Cell reports 2015 Jun 9;11(9):1425-36
Cell reports 2015 Jun 9;11(9):1425-36
ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway.
Adachi S, Homoto M, Tanaka R, Hioki Y, Murakami H, Suga H, Matsumoto M, Nakayama KI, Hatta T, Iemura S, Natsume T
Nucleic acids research 2014 Sep;42(15):10037-49
Nucleic acids research 2014 Sep;42(15):10037-49
Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
Kamon M, Katano M, Hiraki-Kamon K, Hishida T, Nakachi Y, Mizuno Y, Okazaki Y, Suzuki A, Hirasaki M, Ueda A, Nishimoto M, Kato H, Okuda A
Stem cells and development 2014 Sep 15;23(18):2170-9
Stem cells and development 2014 Sep 15;23(18):2170-9
CNOT7/hCAF1 is involved in ICAM-1 and IL-8 regulation by tristetraprolin.
Shi JX, Li JS, Hu R, Shi Y, Su X, Li Q, Zhang F
Cellular signalling 2014 Nov;26(11):2390-6
Cellular signalling 2014 Nov;26(11):2390-6
Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis.
Gou LT, Dai P, Yang JH, Xue Y, Hu YP, Zhou Y, Kang JY, Wang X, Li H, Hua MM, Zhao S, Hu SD, Wu LG, Shi HJ, Li Y, Fu XD, Qu LH, Wang ED, Liu MF
Cell research 2014 Jun;24(6):680-700
Cell research 2014 Jun;24(6):680-700
Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.
Mathys H, Basquin J, Ozgur S, Czarnocki-Cieciura M, Bonneau F, Aartse A, Dziembowski A, Nowotny M, Conti E, Filipowicz W
Molecular cell 2014 Jun 5;54(5):751-65
Molecular cell 2014 Jun 5;54(5):751-65
Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.
Leppek K, Schott J, Reitter S, Poetz F, Hammond MC, Stoecklin G
Cell 2013 May 9;153(4):869-81
Cell 2013 May 9;153(4):869-81
Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs.
Färber V, Erben E, Sharma S, Stoecklin G, Clayton C
Nucleic acids research 2013 Jan;41(2):1211-22
Nucleic acids research 2013 Jan;41(2):1211-22
The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex.
Doidge R, Mittal S, Aslam A, Winkler GS
PloS one 2012;7(12):e51331
PloS one 2012;7(12):e51331
Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.
Zheng X, Dumitru R, Lackford BL, Freudenberg JM, Singh AP, Archer TK, Jothi R, Hu G
Stem cells (Dayton, Ohio) 2012 May;30(5):910-22
Stem cells (Dayton, Ohio) 2012 May;30(5):910-22
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP(CNOT1 antibody) at dilution of 1:800
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The CNOT1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CNOT1. This antibody recognizes human,mouse,rat antigen. The CNOT1 antibody has been validated for the following applications: ELISA, IHC, WB analysis.