13252-1-AP

antibody from Invitrogen Antibodies

Targeting: SRGAP1 ARHGAP13, KIAA1304

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13252-1-AP

Provider

Invitrogen Antibodies

Product name

SRGAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSTPSRFKK DKEIIAEYES QVKEIRAQLV EQQKCLEQQT EMRVQLLQDL QDFFRKKAEI ETEYSRNLEK LAERFMAKTR STKDHQQYKK DQNLLSPVNC WYLLLNQVRR ESKDHATLSD IYLNNVIMRF MQISEDSTRM FKKSKEIAFQ LHEDLMKVLN ELYTVMKTYH MYHAESISAE SKLKEAEKQE EKQIGRSGDP VFHIRLEERH QRRSSVKKIE KMKEKRQAKY SENKLKSIKA RNEYLLTLEA TNASVFKYYI HDLSDLIDCC DLGYHASLNR ALRTYLSAEY NLETSRHEGL D (1-300 aa encoded by BC029919)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 13252-1-AP (SRGAP1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 13252-1-AP (SRGAP1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 13252-1-AP (SRGAP1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 13252-1-AP (SRGAP1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 13252-1-AP) (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SRGAP1 (IP:13252-1-AP, 4ug; Detection:13252-1-AP 1:500) with mouse kidney tissue lysate 4000ug.