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LearnMAB653-100
antibody from R&D Systems
Targeting: CCL26
eotaxin-3, IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB653-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL26/Eotaxin-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CCL11/Eotaxin, recombinant mouse (rm) CCL11/Eotaxin, rhCCL24/Eotaxin-2, rmCCL24/Eotaxin-2, rhCCL3, rhCCL4, rmCCL3, rmCCL4, rhCCL5, rhCCL13, or rhCCL14.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y258- Isotype
- IgG
- Antibody clone number
- 115002
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Miyagaki T, Sugaya M, Fujita H, Ohmatsu H, Kakinuma T, Kadono T, Tamaki K, Sato S
The Journal of investigative dermatology 2010 Sep;130(9):2304-11
The Journal of investigative dermatology 2010 Sep;130(9):2304-11
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Øynebråten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood 2004 Jul 15;104(2):314-20
Blood 2004 Jul 15;104(2):314-20
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CCL26/Eotaxin-3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL26/ Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL26/Eotaxin-3 and Neutralization by Human CCL26/Eotaxin-3 Antibody. Recombinant Human CCL26/Eotaxin-3 (Catalog # 346-E3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653). The ND50 is typically 2-8 µg/mL.
