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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- LS-C815749 - Provider product page
- Provider
- LSBio
- Product name
- FRG1 Antibody (Preservative Free) LS-C815749
- Antibody type
- Polyclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Frg1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C:0.5 mg A549 Whole Cell Lysate. 2 uL anti-Frg1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Frg1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 38 kDa.
