21625-1-AP
antibody from Invitrogen Antibodies
Targeting: RNF20
BRE1, BRE1A, FLJ11189, FLJ20382, hBRE1, KAIA2779
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- 21625-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: YWSQFDENI RIILKRYDLE QGLGDLLTER KALVVPEPEP DSDSNQERKD DRERGEGQEP AFSFLATLAS SSSEEMESQL QERVESSRRA VSQIVTVYDK LQEKVELLSR KLNSGDNLIV EEAVQELNSF LAQENMRLQE LTDLLQEKHR TMSQEFSKLQ SKVETAESRV SVLESMIDDL QWDIDKIRKR EQRLNRHLAE VLERVNSKGY KVYGAGSSLY GGTITINARK FEEMNAELEE NKELAQNRLC ELEKLRQDFE EVTTQNEKLK VELRSAVEQV VKETPEYRCM QSQFSVLYNE SLQLKAHLDE ARTLLHGTRG THQHQVELIE RDEVSLHKK (96-433 aa encoded by BC110585)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat liver tissue were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 21625-1-AP (RNF20 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RNF20 antibody 21625-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using RNF20 antibody 21625-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RNF20 (IP:21625-1-AP, 3ug; Detection:21625-1-AP 1:500) with HeLa cells lysate 2000ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RNF20 (IP:21625-1-AP, 3 ug; Detection:21625-1-AP 1:500) with HeLa cells lysate 2000 ug.