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LearnNB100-2242
antibody from Novus Biologicals
Targeting: RNF20
BRE1, BRE1A, FLJ11189, FLJ20382, hBRE1, KAIA2779
Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-2242 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2242, RRID:AB_2180650
- Product name
- Rabbit Polyclonal RNF20 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy.
Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination.
Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemia.
The epigenetic modifier ubiquitin-specific protease 22 (USP22) regulates embryonic stem cell differentiation via transcriptional repression of sex-determining region Y-box 2 (SOX2).
Promoters active in interphase are bookmarked during mitosis by ubiquitination.
Histone H2B ubiquitin ligases RNF20 and RNF40 in androgen signaling and prostate cancer cell growth.
The tumor suppressor CDC73 interacts with the ring finger proteins RNF20 and RNF40 and is required for the maintenance of histone 2B monoubiquitination.
Nucleosomal H2B ubiquitylation with purified factors.
A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis.
Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1).
Histone ubiquitination associates with BRCA1-dependent DNA damage response.
The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression.
Chen S, Jing Y, Kang X, Yang L, Wang DL, Zhang W, Zhang L, Chen P, Chang JF, Yang XM, Sun FL
Nucleic acids research 2017 Feb 17;45(3):1144-1158
Nucleic acids research 2017 Feb 17;45(3):1144-1158
Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination.
Wu Y, Chen P, Jing Y, Wang C, Men YL, Zhan W, Wang Q, Gan Z, Huang J, Xie K, Mi J, Yu C, Yu X, Chen PC, Chang JF, Cai F, Chen S
PloS one 2015;10(7):e0133444
PloS one 2015;10(7):e0133444
Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemia.
Wang E, Kawaoka S, Yu M, Shi J, Ni T, Yang W, Zhu J, Roeder RG, Vakoc CR
Proceedings of the National Academy of Sciences of the United States of America 2013 Mar 5;110(10):3901-6
Proceedings of the National Academy of Sciences of the United States of America 2013 Mar 5;110(10):3901-6
The epigenetic modifier ubiquitin-specific protease 22 (USP22) regulates embryonic stem cell differentiation via transcriptional repression of sex-determining region Y-box 2 (SOX2).
Sussman RT, Stanek TJ, Esteso P, Gearhart JD, Knudsen KE, McMahon SB
The Journal of biological chemistry 2013 Aug 16;288(33):24234-46
The Journal of biological chemistry 2013 Aug 16;288(33):24234-46
Promoters active in interphase are bookmarked during mitosis by ubiquitination.
Arora M, Zhang J, Heine GF, Ozer G, Liu HW, Huang K, Parvin JD
Nucleic acids research 2012 Nov 1;40(20):10187-202
Nucleic acids research 2012 Nov 1;40(20):10187-202
Histone H2B ubiquitin ligases RNF20 and RNF40 in androgen signaling and prostate cancer cell growth.
Jääskeläinen T, Makkonen H, Visakorpi T, Kim J, Roeder RG, Palvimo JJ
Molecular and cellular endocrinology 2012 Mar 5;350(1):87-98
Molecular and cellular endocrinology 2012 Mar 5;350(1):87-98
The tumor suppressor CDC73 interacts with the ring finger proteins RNF20 and RNF40 and is required for the maintenance of histone 2B monoubiquitination.
Hahn MA, Dickson KA, Jackson S, Clarkson A, Gill AJ, Marsh DJ
Human molecular genetics 2012 Feb 1;21(3):559-68
Human molecular genetics 2012 Feb 1;21(3):559-68
Nucleosomal H2B ubiquitylation with purified factors.
Kim J, Roeder RG
Methods (San Diego, Calif.) 2011 Jul;54(3):331-8
Methods (San Diego, Calif.) 2011 Jul;54(3):331-8
A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis.
Taniue K, Oda T, Hayashi T, Okuno M, Akiyama T
EMBO reports 2011 Jul 1;12(7):682-9
EMBO reports 2011 Jul 1;12(7):682-9
Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1).
Buro LJ, Chipumuro E, Henriksen MA
Epigenetics & chromatin 2010 Sep 8;3(1):16
Epigenetics & chromatin 2010 Sep 8;3(1):16
Histone ubiquitination associates with BRCA1-dependent DNA damage response.
Wu J, Huen MS, Lu LY, Ye L, Dou Y, Ljungman M, Chen J, Yu X
Molecular and cellular biology 2009 Feb;29(3):849-60
Molecular and cellular biology 2009 Feb;29(3):849-60
The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression.
Shema E, Tirosh I, Aylon Y, Huang J, Ye C, Moskovits N, Raver-Shapira N, Minsky N, Pirngruber J, Tarcic G, Hublarova P, Moyal L, Gana-Weisz M, Shiloh Y, Yarden Y, Johnsen SA, Vojtesek B, Berger SL, Oren M
Genes & development 2008 Oct 1;22(19):2664-76
Genes & development 2008 Oct 1;22(19):2664-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF20 Antibody [NB100-2242] - WB analysis of RNF20 in HeLa whole cell lysate. Image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human RNF20 on HeLa whole cell lysate using NB100-2242. RNF20 was also immunoprecipitated using another rabbit anti-RNF20 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RNF20 Antibody [NB100-2242] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody NB100-2242 (lot NB100-2242-2) used for IP at 6 ug per reaction. RNF20 was also immunoprecipitated by a previous lot of this antibody (NB100-2242-1) and rabbit anti-RNF20 antibody NB100-2243. For blotting immunoprecipitated RNF20, NB100-2242 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
