Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-84814 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LQRAEEAPRR QLRVSQRTDG ESRAHLGALL ARYIQQARKA PSGRMSIVKN LQNLDPSHRI SDRDYMG
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localization.
Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis.
Chen X, Lu Y, Guo G, Zhang Y, Sun Y, Guo L, Li R, Nan Y, Yang X, Dong J, Jin X, Huang Q
Oncology reports 2021 Jul;46(1)
Oncology reports 2021 Jul;46(1)
Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis.
Zhu L, Chen R, Jiang C, Xie Q, Zhao W, Gao X, Huang H
Molecular medicine reports 2021 Aug;24(2)
Molecular medicine reports 2021 Aug;24(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCK in human duodenum and skeletal muscle tissues using a CCK polyclonal antibody (Product # PA5-84814). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCK in human duodenum using a CCK polyclonal antibody (Product # PA5-84814). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCK in human hypothalamus using a CCK polyclonal antibody (Product # PA5-84814). The analysis shows strong positivity in neuronal projections.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCK in human skeletal muscle using a CCK polyclonal antibody (Product # PA5-84814). The analysis shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. AMOTL2 inhibits the proliferation, migration and invasion of glioma cells. (A and B) The U87MG cell line was transfected with lentiviral vectors of AMOTL2 shRNA and overexpressed plasmids of AMOTL2. Reverse transcription-quantitative PCR and western blotting were performed to examine the AMOTL2 expression level with control and treatment groups. (C) Colony formation assays were conducted using the U87MG cell line to examine the effect of the expression of AMOTL2 in cell colony formation (n=3). (D) The effect of AMOTL2 on proliferation was examined by Cell Counting Kit-8 assay in U87MG and U251 cell lines (n=3). (E) The migrating ability of U87MG cells was detected by wound healing assay (n=5). (F) Transwell migration (upper images) and Matrigel (r) invasion (lower images) assays were conducted to investigate the effect of AMOTL2 knockdown on U87MG cell migration and invasion (n=5). Results are presented as the mean +- SD. *P