Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA500796 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LEMD3 Monoclonal Antibody (OTI3H1), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3H1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-LEMD3 mouse monoclonal antibody (TA500796) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3(RC212759).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-LEMD3 antibody (TA500796), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of LEMD3 by using TrueMab monoclonal anti-LEMD3 antibody (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-LEMD3 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
