A302-860A

antibody from Invitrogen Antibodies

Targeting: RTN3 ASYIP, HAP, NSPL2, NSPLII, RTN3-A1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-860A

Provider

Invitrogen Antibodies

Product name

Reticulon-3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Reticulon-3 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-Reticulon-3 antibody (Product # A302-860A; lot A302-860A-1) used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Reticulon-3 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Reticulon-3 antibody A302-860A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Reticulon-3 was also immunoprecipitated by rabbit anti-Reticulon-3 antibody A302-859A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Reticulon-3 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Reticulon-3 (Cat. No. A302-860A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Reticulon-3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Reticulon-3 antibody (Product # A302-860A; lot A302-860A-1) used for IP at 3 µg/mg lysate. Reticulon-3 was also immunoprecipitated by rabbit anti-Reticulon-3 antibody (Product # A302-859A), which recognizes an upstream epitope. For blotting immunoprecipitated Reticulon-3 (Product # A302-860A) was used at 0.4 µg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.