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LearnPA5-109213
antibody from Invitrogen Antibodies
Targeting: DDIT4
Dig2, FLJ20500, REDD-1, REDD1, RTP801
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- PA5-109213 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDIT4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Hela and K562 cell lysates, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, A549.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDIT4 in HeLa (1) and K562 (2) cell lysates. Samples were incubated with DDIT4 polyclonal antibody (Product # PA5-109213), at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDIT4 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with DDIT4 polyclonal antibody (Product # PA5-109213), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDIT4 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with DDIT4 polyclonal antibody (Product # PA5-109213), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDIT4 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with DDIT4 polyclonal antibody (Product # PA5-109213), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DDIT4 in paraffin-embedded rat epididymis tissue. Samples were incubated with DDIT4 polyclonal antibody (Product # PA5-109213), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DDIT4 in paraffin-embedded mouse colon tissue. Samples were incubated with DDIT4 polyclonal antibody (Product # PA5-109213), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DDIT4 in paraffin-embedded human tonsil tissue. Samples were incubated with DDIT4 polyclonal antibody (Product # PA5-109213), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DDIT4 in paraffin-embedded human colon cancer tissue. Samples were incubated with DDIT4 polyclonal antibody (Product # PA5-109213), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DDIT4 in paraffin-embedded human breast tissue. Samples were incubated with DDIT4 polyclonal antibody (Product # PA5-109213), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of DDIT4 in A549 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with DDIT4 polyclonal antibody (Product # PA5-109213) at a dilution of 1:100.
