PA5-13253

antibody from Invitrogen Antibodies

Targeting: DDIT4 Dig2, FLJ20500, REDD-1, REDD1, RTP801

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13253

Provider

Invitrogen Antibodies

Product name

DDIT4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, mouse and rat based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The Hydroxyquinoline Analogue YUM70 Inhibits GRP78 to Induce ER Stress-Mediated Apoptosis in Pancreatic Cancer.

Samanta S, Yang S, Debnath B, Xue D, Kuang Y, Ramkumar K, Lee AS, Ljungman M, Neamati N
Cancer research 2021 Apr 1;81(7):1883-1895

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDIT4 (arrow) using a DDIT4 polyclonal antibody (Product # PA5-13253) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DDIT4 gene.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human pancreas tissue using a DDIT4 polyclonal antibody (Product # PA5-13253), followed by HRP-conjugated secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of 293 cells using a DDIT4 polyclonal antibody (Product # PA5-13253) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody