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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-28261 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAGLU Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Predicted reactivity: Mouse (86%), Bovine (89%).
- Concentration
- 0.69 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAGLU using 30 µg of A) H1299 (B) HeLa S3 and C) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NAGLU polyclonal antibody (Product # PA5-28261) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NAGLU was performed in U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU Polyclonal Antibody (Product # PA5-28261) diluted at 1:250. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NAGLU was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU Polyclonal Antibody (Product # PA5-28261) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NAGLU was performed in paraffin-embedded mouse brain tissue using NAGLU Polyclonal Antibody (Product # PA5-28261) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NAGLU was performed in paraffin-embedded rat duodenum tissue using NAGLU Polyclonal Antibody (Product # PA5-28261) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NAGLU was performed in paraffin-embedded mouse pancreas tissue using NAGLU Polyclonal Antibody (Product # PA5-28261) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NAGLU was performed in paraffin-embedded rat pancreas tissue using NAGLU Polyclonal Antibody (Product # PA5-28261) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of NAGLU was performed in frozen-sectioned adult mouse cerebellum tissue using NAGLU Polyclonal Antibody (Product # PA5-28261) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NAGLU was performed in paraffin-embedded rat brain tissue using Green: alpha Catenin Polyclonal Antibody (Product # PA5-29247) at a dilution of 1:200. Blue: Nuclear staining with Hoechst 33342. The signal was developed using a goat anti-rabbit IgG antibody.
