PA5-30097

antibody from Invitrogen Antibodies

Targeting: NAGLU NAG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30097

Provider

Invitrogen Antibodies

Product name

NAGLU Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NAGLU using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NAGLU polyclonal antibody (Product # PA5-30097) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NAGLU was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NAGLU Polyclonal Antibody (Product # PA5-30097) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NAGLU was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NAGLU Polyclonal Antibody (Product # PA5-30097) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NAGLU was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NAGLU Polyclonal Antibody (Product # PA5-30097) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NAGLU was performed in U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU Polyclonal Antibody (Product # PA5-30097) diluted at 1:200. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of NAGLU was performed in paraffin-embedded mouse brain tissue using NAGLU Polyclonal Antibody (Product # PA5-30097) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NAGLU (Product # PA5-30097) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.