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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PB9599 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-5HT2A Receptor/HTR2A Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for 5 HT2A/HTR2A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. 5 HT2A/HTR2A information: Molecular Weight: 52603 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein. Cell projection, dendrite . Cell projection, axon . Cytoplasmic vesicle . Membrane, caveola . Localizes to the postsynaptic thickening of axo-dendritic synapses; Tissue Specificity: Detected in brain cortex (at protein level). Detected in blood platelets.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Regulatory Effect of Electroacupuncture on Hypothalamic Serotonin and its Receptor in Rats with Cerebral Ischemia.
Histone deacetylase inhibitors mitigate antipsychotic risperidone-induced motor side effects in aged mice and in a mouse model of Alzheimer's disease.
Fluoxetine attenuates chronic methamphetamine-induced pulmonary arterial remodelling: possible involvement of serotonin transporter and serotonin 1B receptor.
Ma T, Li C, Nie Z, Miao H, Wu F
Current neurovascular research 2023;20(2):237-243
Current neurovascular research 2023;20(2):237-243
Histone deacetylase inhibitors mitigate antipsychotic risperidone-induced motor side effects in aged mice and in a mouse model of Alzheimer's disease.
Rodriguez G, Fisher DW, McClarty B, Montalvo-Ortiz J, Cui Q, Chan CS, Dong H
Frontiers in psychiatry 2022;13:1020831
Frontiers in psychiatry 2022;13:1020831
Fluoxetine attenuates chronic methamphetamine-induced pulmonary arterial remodelling: possible involvement of serotonin transporter and serotonin 1B receptor.
Liu M, Wang Y, Wang HM, Bai Y, Zhang XH, Sun YX, Wang HL
Basic & clinical pharmacology & toxicology 2013 Feb;112(2):77-82
Basic & clinical pharmacology & toxicology 2013 Feb;112(2):77-82
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of 5HT2A Receptor using anti-5HT2A Receptor antibody (PB9599). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2A Receptor antigen affinity purified polyclonal antibody (Catalog # PB9599) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2A Receptor at approximately 53KD. The expected band size for 5HT2A Receptor is at 53KD.
- Additional image
