PB9599

antibody from Boster Biological Technology

Targeting: HTR2A 5-HT2A, HTR2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PB9599

Provider

Boster Biological Technology

Product name

Anti-5HT2A Receptor/HTR2A Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for 5 HT2A/HTR2A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. 5 HT2A/HTR2A information: Molecular Weight: 52603 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein. Cell projection, dendrite . Cell projection, axon . Cytoplasmic vesicle . Membrane, caveola . Localizes to the postsynaptic thickening of axo-dendritic synapses; Tissue Specificity: Detected in brain cortex (at protein level). Detected in blood platelets.

Reactivity

Human, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of 5HT2A Receptor using anti-5HT2A Receptor antibody (PB9599). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2A Receptor antigen affinity purified polyclonal antibody (Catalog # PB9599) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2A Receptor at approximately 53KD. The expected band size for 5HT2A Receptor is at 53KD.

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