Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ASR-033-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 5HT2A Receptor (HTR2A) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.9 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat DRG (lanes 1 and 4), mouse brain (lanes 2 and 5) and human brain neuroblastoma SH-SY5Y (lanes 3 and 6)lysates: - 1-3. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:200).4-6. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody , preincubated with 5HT2A Receptor/HTR2A (extracellular) Blocking Peptide (#BLP-SR033).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat DRG (lanes 1 and 4), mouse brain (lanes 2 and 5) and human brain neuroblastoma SH-SY5Y (lanes 3 and 6)lysates: - 1-3. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:200).4-6. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody , preincubated with 5HT2A Receptor/HTR2A (extracellular) Blocking Peptide (#BLP-SR033).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of5HT2A Receptorin ratcingulatecortex. Immunohistochemicalstaining of perfusion-fixed frozen rat brain sections with Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:1200), followed by goat Anti-rabbit-AlexaFluor-488.A.5HT2A Receptorimmunoreactivity (green) appearsinoutlines of cortical neurons (arrows).B. Pre-incubation of the Antibody with 5HT2A Receptor (HTR2A) (extracellular)Blocking Peptide (BLP-SR033), suppressed staining. Cell nucleiare stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of5HT2A Receptorin ratdorsal raphe nucleus. Immunohistochemicalstaining of perfusion-fixed frozen rat brain sections with Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:1200), followed by goat Anti-rabbit-AlexaFluor-488.A. 5HT2A Receptorimmunoreactivity (green) appearsinoutlines of neurons (arrows).B. Pre-incubation of the Antibody with 5HT2A Receptor (HTR2A) (extracellular)Blocking Peptide (BLP-SR033), suppressed staining. Cell nucleiare stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of 5HT2A Receptor (HTR2A) by indirect flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (5 µg) + goat- Anti-rabbit-FITC.
