AF4186

antibody from Novus Biologicals

Targeting: MLX bHLHd13, MAD7, MXD7, TCFL4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF4186

Provider

Novus Biologicals

Product name

Goat Polyclonal MLX Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human MLX in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Thioredoxin-interacting protein gene expression via MondoA is rapidly and transiently suppressed during inflammatory responses.

Kanari Y, Sato Y, Aoyama S, Muta T
PloS one 2013;8(3):e59026

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human MLX by Western Blot. Western blot shows nuclear extracts of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MLX at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.