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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-92301 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92301, RRID:AB_11002278
- Product name
- Rabbit Polyclonal PSMC3IP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat PSMC3IP antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Break-induced telomere synthesis underlies alternative telomere maintenance.
Breast cancer genes PSMC3IP and EPSTI1 play a role in apoptosis regulation.
Interchromosomal homology searches drive directional ALT telomere movement and synapsis.
Dilley RL, Verma P, Cho NW, Winters HD, Wondisford AR, Greenberg RA
Nature 2016 Nov 3;539(7627):54-58
Nature 2016 Nov 3;539(7627):54-58
Breast cancer genes PSMC3IP and EPSTI1 play a role in apoptosis regulation.
Capdevila-Busquets E, Badiola N, Arroyo R, Alcalde V, Soler-López M, Aloy P
PloS one 2015;10(1):e0115352
PloS one 2015;10(1):e0115352
Interchromosomal homology searches drive directional ALT telomere movement and synapsis.
Cho NW, Dilley RL, Lampson MA, Greenberg RA
Cell 2014 Sep 25;159(1):108-121
Cell 2014 Sep 25;159(1):108-121
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PSMC3IP Antibody [NBP1-92301] - Simple Western lane view shows a specific band for PSMC3IP in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PSMC3IP Antibody [NBP1-92301] - Simple Western lane view shows a specific band for PSMC3IP in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PSMC3IP Antibody [NBP1-92301] - Electropherogram image(s) of corresponding Simple Western lane view. PSMC3IP antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSMC3IP Antibody [NBP1-92301] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSMC3IP Antibody [NBP1-92301] - Analysis in human testis tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PSMC3IP Antibody [NBP1-92301] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PSMC3IP Antibody [NBP1-92301] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PSMC3IP Antibody [NBP1-92301] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PSMC3IP Antibody [NBP1-92301] - Staining in human testis and liver tissues using anti-PSMC3IP antibody. Corresponding PSMC3IP RNA-seq data are presented for the same tissues.
