Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21907 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HRH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH. Predicted reactivity: Rhesus Monkey (100%), Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histamine H2 Receptor using A) 30 µg U87-MG whole cell lysate and B) 30 µg SK-N-SH whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Histamine H2 Receptor polyclonal antibody (Product # PA5-21907) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HRH2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HRH2 Polyclonal Antibody (Product # PA5-21907) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HRH2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HRH2 Polyclonal Antibody (Product # PA5-21907) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HRH2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HRH2 Polyclonal Antibody (Product # PA5-21907) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Histamine H2 Receptor (Product # PA5-21907) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
