PA5-21907

antibody from Invitrogen Antibodies

Targeting: HRH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21907

Provider

Invitrogen Antibodies

Product name

HRH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH.

Concentration

0.75 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Histamine H2 Receptor using A) 30 µg U87-MG whole cell lysate and B) 30 µg SK-N-SH whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Histamine H2 Receptor polyclonal antibody (Product # PA5-21907) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HRH2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HRH2 Polyclonal Antibody (Product # PA5-21907) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HRH2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HRH2 Polyclonal Antibody (Product # PA5-21907) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HRH2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HRH2 Polyclonal Antibody (Product # PA5-21907) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Histamine H2 Receptor (Product # PA5-21907) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.