Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-812 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-812, RRID:AB_2057804
- Product name
- Goat Polyclonal Histamine H2R Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms.
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Histamine deficiency promotes inflammation-associated carcinogenesis through reduced myeloid maturation and accumulation of CD11b+Ly6G+ immature myeloid cells
Histamine: its novel role as an endogenous regulator of Con A-dependent T cell proliferation.
Xiang Dong Yang, Walden Ai, Samuel Asfaha, Govind Bhagat, Richard A Friedman, Guangchun Jin, Heuijoon Park, Benjamin Shykind, Thomas G Diacovo, Andras Falus, Timothy C Wang
Nature Medicine 2010 Dec;17(1):87-95
Nature Medicine 2010 Dec;17(1):87-95
Histamine: its novel role as an endogenous regulator of Con A-dependent T cell proliferation.
Nakane H, Sonobe Y, Watanabe T, Nakano K
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2004 Jul;53(7):324-8
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2004 Jul;53(7):324-8
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histamine H2R Antibody [NB600-812] - Staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Histamine H2R Antibody [NB600-812] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
