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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2845 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2845, RRID:AB_1575581
- Product name
- NAE1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NAE1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
ASPP2 inhibits APP-BP1-mediated NEDD8 conjugation to cullin-1 and decreases APP-BP1-induced cell proliferation and neuronal apoptosis.
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Chen Y, Liu W, McPhie DL, Hassinger L, Neve RL
The Journal of cell biology 2003 Oct 13;163(1):27-33
The Journal of cell biology 2003 Oct 13;163(1):27-33
ASPP2 inhibits APP-BP1-mediated NEDD8 conjugation to cullin-1 and decreases APP-BP1-induced cell proliferation and neuronal apoptosis.
Chen Y, Liu W, Naumovski L, Neve RL
Journal of neurochemistry 2003 May;85(3):801-9
Journal of neurochemistry 2003 May;85(3):801-9
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA
Nature 2003 Mar 20;422(6929):330-4
Nature 2003 Mar 20;422(6929):330-4
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NAE1 (arrow) using rabbit NAE1 polyclonal antibody (Cat # PAB2845). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NAE1 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NAE1 polyclonal antibody (Cat # PAB2845) in mouse brain tissue lysates (35 ug/lane). NAE1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NAE1 polyclonal antibody (Cat # PAB2845) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
