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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [5]
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- Product number
- PA5-59836 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KPFPELREHF QSYDLDHMEK KDHSHTPWIV IIAKYLAQWY SETNGRIPKT YKEKEDFRDL IRQGILKNEN GAPEDEENFE EAIKNVNTAL NTTQIPSSIE DIFNDDR Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 98%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Organoid-based drug screening reveals neddylation as therapeutic target for malignant rhabdoid tumors.
Calandrini C, van Hooff SR, Paassen I, Ayyildiz D, Derakhshan S, Dolman MEM, Langenberg KPS, van de Ven M, de Heus C, Liv N, Kool M, de Krijger RR, Tytgat GAM, van den Heuvel-Eibrink MM, Molenaar JJ, Drost J
Cell reports 2021 Aug 24;36(8):109568
Cell reports 2021 Aug 24;36(8):109568
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAE1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a NAE1 Polyclonal Antibody (Product # PA5-59836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nae1 was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a polyclonal anti-Nae1 antibody (Product # PA5-59836) at a dilution of 1:500 in 5% milk/TBST at 4oC overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:10000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209). Data courtesy of Thermo Scientific KOL Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NAE1 using NAE1 Polyclonal Antibody (Product # PA5-59836) (A) shows similar pattern to an independent NAE1 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NAE1 in human cerebral cortex using NAE1 Polyclonal Antibody (Product # PA5-59836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NAE1 in human testis using NAE1 Polyclonal Antibody (Product # PA5-59836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NAE1 in human liver using NAE1 Polyclonal Antibody (Product # PA5-59836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NAE1 in human colon using NAE1 Polyclonal Antibody (Product # PA5-59836).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NAE1 in human cerebellum using a NAE1 Polyclonal Antibody (Product # PA5-59836) shows strong nuclear positivity in Purkinje cells.
