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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 10192-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LAMSPGEEI LTLLQTIPFD IEDLKKEAAN LPPEDDDQWL DLSPDQLDQL LQEAVGKKES ESVSKEEKEQ NYDLTEVSES MKAFISKVST HKGAELPREP SEAPITFDAD SFLNYFDKIL GPRPNESDSD DLDDEDFECL DSDDDLDFET HEPGEEASLK GTLDNLKSYM AQMDQELAHT CISKSFTTRN QVEPVSQTTD NNSDEEDSGT GESVMAPVDV DLNLVSNILE SYSSQAGLAG PASNLLQSMG VQLPDNTDHR PTSKP (378-641 aa encoded by BC000721)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 10192-1-AP (ECD antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ECD (IP:10192-1-AP, 4ug; Detection:10192-1-AP 1:300) with HeLa cells lysate 2480ug.
