12110-1-AP

antibody from Invitrogen Antibodies

Targeting: PADI2 KIAA0994, PDI2

Provider product page for 12110-1-AP

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [6]
Immunocytochemistry [4]
Immunohistochemistry [10]
Other assay [1]

Submit

Product number: 12110-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: PADI2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: ISLDVDADR DGVVEKNNPK KASWTWGPEG QGAILLVNCD RETPWLPKED CRDEKVYSKE DLKDMSQMIL RTKGPDRLPA GYEIVLYISM SDSDKVGVFY VENPFFGQRY IHILGRRKLY HVVKYTGGSA ELLFFVEGLC FPDEGFSGLV SIHVSLLEYM AQDIPLTPIF TDTVIFRIAP WIMTPNILPP VSVFVCCMKD NYLFLKEVKN LVEKTNCELK VCFQYLNRGD RWIQDEIEFG YIEAPHKGFP VVLDSPRDGN LKDFPVKELL GPDFGYVTRE PLFESVTSLD SFGNLEVSPP VTVNGKTYPL GRILIGSSFP L (118-438 aa encoded by BC009701)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.22 mg/mL
Storage: -20°C

PADI2 protein structure - 12110-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF-7 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF-7 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat brain tissue were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human gliomas using 12110-1-AP (PADI2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human kidney using 12110-1-AP (PADI2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human kidney using 12110-1-AP (PADI2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer using 12110-1-AP (PADI2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer using 12110-1-AP (PADI2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12110-1-AP ( PADI2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12110-1-AP ( PADI2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).