GTX132110

antibody from GeneTex

Targeting: HMGA1 HMGIY

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX132110

Provider

GeneTex

Product name

HMGA1 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:3000.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod no

Proprietary.

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

Antibody Lot Number

42270

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:3000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA1 antibody detects HMGA1 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA1 protein stained by HMGA1 antibody (GTX132110) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.