H00001831-M01

antibody from Abnova Corporation

Targeting: TSC22D3 DIP, DSIPI, GILZ, hDIP, TSC-22R

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001831-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001831-M01, RRID:AB_607241

Product name

TSC22D3 monoclonal antibody (M01), clone 3A5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant TSC22D3.

Antigen sequence

MAQSKLDCRSPVGLDCCNCCLDLAHRSGLQRGSSG
ENNNPGSPTVSNFRQLQEKLVFENLNTDKLNSIMR
QDSLEPVLRDPCYLINEGICNRNIDQT


Isotype

IgG

Antibody clone number

3A5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody (M01), clone 3A5.Lane 1: TSC22D3 transfected lysate(22.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of TSC22D3 transfected lysate using anti-TSC22D3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TSC22D3 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol