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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- H00001831-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001831-M01, RRID:AB_548879
- Product name
- Mouse Monoclonal GilZ Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TSC22D3 - TSC22 domain family, member 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody (M01), clone 3A5.Lane 1: TSC22D3 transfected lysate(22.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GilZ Antibody (3A5) [H00001831-M01] - Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 transfected lysate using anti-TSC22D3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TSC22D3 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Human lymph node. Antigen retrieval in citrate buffer at 95C for 30 min. Image from verified customer review.
