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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG54153 - Provider product page
- Provider
- Arigo
- Product name
- anti-hHR23b antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human hHR23b protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5H1-A10-A7
- Vial size
- 100 µl
- Concentration
- 1.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: hHR23b in A431, K562, Jurkat, C6, 3T3 and HeLa cell lysates stained with hHR23b antibody [5H1-A10-A7] (ARG54153) (diluted in 1: 1000). Predicted band size: 58KDa. Observed band size: 58KDa. Exposure time: 5min.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: hHR23b in K562, SW480, CHO-K1, 3T3 and COS7 cell lysates stained with hHR23b antibody [5H1-A10-A7] (ARG54153) (diluted in 1: 1000). Predicted band size: 58KDa. Observed band size: 58KDa. Exposure time: 5min.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with hHR23b antibody [5H1-A10-A7] (ARG54153) (diluted in 1:100).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Prostate Cancer stained with hHR23b antibody [5H1-A10-A7] (ARG54153) (diluted in 1/100). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6. 0).
