BS-10348R

antibody from Invitrogen Antibodies

Targeting: HAMP HEPC, HFE2B, LEAP-1, LEAP1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BS-10348R

Provider

Invitrogen Antibodies

Product name

Hepcidin 25 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin-embedded Human Liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Hepcidin 25 Polyclonal Antibody, Unconjugated (bs-10348R) at 1:400 overnight at 4°C, followed by secondary antibody and DAB staining.