Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B2277 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B2277, RRID:AB_2250651
- Product name
- IHC-plus™ TRIM1 / MID2 Antibody (aa723-735) LS-B2277
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Antibody (1 ug/ml) staining of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-MID2 / TRIM1 Antibody (0.1µg/ml) staining of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.