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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6327 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6327, RRID:AB_1577286
- Product name
- MID2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of MID2.
- Antigen sequence
QESPYVSGMKTCH- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development.
Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B
Human molecular genetics 1999 Aug;8(8):1397-407
Human molecular genetics 1999 Aug;8(8):1397-407
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MID2 polyclonal antibody (Cat # PAB6327) staining (1 ug/mL) of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MID2 polyclonal antibody (Cat # PAB6327, 5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
