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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Chromatin Immunoprecipitation [2]
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- Product number
- ABIN2616407 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Anoctamin 10 (ANO10) (AA 1-393) antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-393
- Isotype
- IgG
- Antibody clone number
- 2B12-1A11
- Vial size
- 100 μg
- Storage
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
- Handling
- Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 acetyl Lys56 antibody tested by Western blot. HeLa acid extract (10 μg/lane) was probed with Histone H3 acetyl Lys56 polyclonal antibody (1:1,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.
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- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 acetyl Lys56 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys56 pAb for acetyl Lys56 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:20,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H3 acetyl-Lys4 peptide. Lane 2: H3 acetyl-Lys9 peptide. Lane 3: H3 acetyl-Lys14 peptide. Lane 4: H3 acetyl-Lys18 peptide.. Lane 5: unmodified H3 peptide aa 13-22. Lane 6: H3 acetyl-Lys23 peptide. Lane 7: H3 acetyl-Lys27 peptide. Lane 8: H3 acetyl-Lys36 peptide. Lane 9: H3 acetyl-Lys37 peptide. Lane 10: yeast H3 acetyl-Lys56 peptide. Lane 11: unmodified yeast H3 peptide aa 50-59. Lane 12: human H3 acetyl-Lys56 peptide.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 acetyl Lys56 antibody tested by Western blot. Acid extract of yeast cells probed with Histone H3 acetyl Lys56 pAb (1:1,400 dilution).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3K56ac antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million HeLa cells and 5 μl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image on the left shows H3K56Ac binding across a 650,000 bp region on chromosome 17. The image on the right shows H3K56Ac binding at the KLF5 gene.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Histone H3 acetyl Lys56 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 2 μl of Histone H3 acetyl Lys56 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
