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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX105986 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105986, RRID:AB_11172858
- Product name
- GABA B Receptor 2 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX105986 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GABA B Receptor 2 antibody [N2C1], Internal (GTX105986) diluted at 1:2000 and DDDDK tag antibody (GTX115043) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GABA B Receptor 2 antibody [N2C1], Internal detects GABA B Receptor 2 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: GABA B Receptor 2 protein stained by GABA B Receptor 2 antibody [N2C1], Internal (GTX105986) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
